Showing posts with label science. Show all posts
Showing posts with label science. Show all posts

Saturday, March 15, 2014

My Bio Professor Rocks My Socks Off (Some of My Classmates Do Not)

I have her for both the lab and lecture portions of General Biology II. That was totally an accident but a very happy one. I like having the same professor for both lab and lecture (if he or she is good) because I don't have that frustrating situation where one of them say, "I don't know if you've covered this in lab/lecture or not yet, but here we go anyway ..." Also, I have the added bonus of seeing them three times per week and really enjoying all the time I'm actually spending in class.

During our very first lab session she said, "If you can't make it to our Tuesday night lab, I also teach identical sections on Wednesday and Thursday afternoons. Please feel free to make up your time then. If that doesn't work, e-mail any other professor teaching this lab and ask to sit in their session. I will not mind at all." I think she has this perspective because, as I've come to find out, she was/is a non-traditional student herself. She, too, attended this same school a dozen years ago while raising a child and continues to work now toward a PhD. She knows how difficult it is to balance a family, work, and school all at once.

In other classes, lab instructors have allowed for the making up of only one session this way. It's nice to have a semester where that isn't a problem. Consequently, I've made up three sessions this way already this semester and one of them was a lab practical.

Going to these other lab sessions has allowed me to meet a few students who attend during daytime classes. They are, generally speaking, um ... an interesting bunch. Let me explain.

I have heard several professors allude to enjoying teaching nighttime or Saturday morning classes because the students in those classes are more mature, tend to be extremely dedicated, and take their classes seriously. I hadn't given it much thought until meeting these other students. Now I know of what these professors speak.

In the first make up session I attended, I sat in an empty seat at a lab table with three other students. The table held a dozen agar plates for that day's experiment. One guy immediately picked up an agar plate, opened it, and began sniffing the agar. When the professor explained that each plate should be labeled a certain way, he again took off each lid and began writing on the plates. And he's one of those people who, when concentrating hard, sticks his tongue out. A lot. With the agar plate about 2 inches from his face.

I was cringing the entire time. One does not remove agar plate lids until absolutely  necessary. One does not place agar plates near one's face. Ever. Hello, contamination, anyone?! I didn't want to say anything though, because I was crashing their lab group. Instead, I sat and cringed and tried to pretend it wasn't happening. Finally, a TA noticed him and told him politely to stop immediately.

Later, during that same lab, another guy at the table sterilized an inoculating loop in heat, then set it down on the table top. A minute later he picked it up and was about to use it without sterilizing it again. I stopped him, saying that it had to be sterilized. He said, "But I just did that." I replied, "Yes, but then it touched the table again." He rolled his eyes at me. I just stared at him until he put it back into the heat. Geez.

This past week I attended a different lab session so I could take my first practical exam. Practical exams are not group activities and they usually require moving about stations which are set up around the room. Where one sits is completely irrelevant. I sat down at a table with one other guy and two empty seats. He gave me A Look and said, "Um, actually, someone already sits there." I said, "Oh, really? Hm. Thanks, but I don't think it really matters during a practical." Sure enough, it didn't matter.

I hope this kind of student either, a) is not majoring in biology or medicine or chemistry or anything else requiring precision, or b) if they are, they learn the importance of sterile technique and letting little things go. And no, sterile technique is, most definitely, not a little thing.

Anyway, my professor rocks. In addition to understanding the difficulty I am facing as a student/worker/parent, she is funny and has an effective and engaging teaching style. This is her last semester teaching here and I'm sad that I won't have the opportunity to take any more of her classes.

And, because I don't have any other way to end this post, here is a photo of  Ceratopteris cornuta. It's a type of aquatic fern. It's the fern we were studying during the lab session where that one guy sullied our agar plates and that other guy almost sullied our specimens of Ceratopteris cornuta.

 


The purpose of the lab was to sow spores from this fern onto several agar plates using a serial dilution process. We were looking at the relationship between total number of spores and gender distribution of spores. Did you know that this particular fern germinates male and hermaphrodite gametophytes? They do. The hermaphrodite gametophytes release a pheromone called antheridiogen which signals as yet ungerminated spores to become males. When there are many spores in a given area, there are a high number of them which are male. This is because the hermaphrodites can fertilize themselves if necessary but that isn't desirable. What is desirable is having plenty of males around for cross fertilization which brings about genetic diversity. Science is pretty cool.

Tuesday, August 23, 2011

Earth Weirdness

Last night we got home from the Astros' game about 11:00 and the kids went straight to bed.  I did too but I didn't sleep right away.  I have to catch up on Words with Friends you know.  That takes a while.  Plus, I just updated Angry Birds Rio and this requires that I attempt all levels as soon as possible.

So there I was, lying in bed freeing little birdies, killing marmosets and constructing words I didn't know were words until WWF came along.  I was just starting to feel sleepy and about to shut off my phone when my bed moved.  I am not kidding.  It was a very slow, steady movement back and forth, 3 times.  At first I thought one of the kids was climbing onto my bed.  They weren't.

And then.  Then.  Then my hyperactive imagination went straight to the Boogey Man.  Don't laugh.  I had this fear as a kid that there was a Boogey Man under my bed that would grab my ankles when I tried to go to the bathroom or something.  For that reason, I always leapt off my bed a good 2 feet or so when I had to pee in the middle of the night.

So what did I do last night?  I leapt off my bed, turned on the light and flipped up the dust ruffle, golf club in hand to smash the Boogey Man in the face.  There was no Boogey Man. Whew, I dodged a bullet there.

I lay back down wondering what in the world would have caused my bed to move like that.  And I had this fleeting thought, "What if it was an earthquake?  Is that even possible?" And then I laughed at myself. That's about as likely as the Boogey Man scenario, I thought.  And then I went to sleep.

But wait! We did actually have an earthquake in Colorado last night. Its epicenter was near Trinidad, which is one of the two towns where the ex-husband and I meet to trade off the kids. Apparently people felt it a good 75 miles north of here, even. So weird.  Maybe I need to re-think the likelihood of this Boogey Man.

And there was another earthquake, a bit bigger, near Washington, D.C. today. What must the incredible odds be of earthquakes as big as these in two places so unused to having earthquakes? In the same 24 hour period? I'm telling you, this is serious weirdness.

Tuesday, February 1, 2011

Cellular Respiration

Cells need energy.  To generate this energy they go through several complicated processes in the mitochondria of living cells.  (In the mitochondria of eukaryotic animal cells, that is.  Prokaryotic cells do not have mitochondria.  And eukaryotic plant cells do something else but we haven't talked about that yet.)

The first is glycolysis which occurs in the cytoplasm of cells.  Glycolysis literally means "splitting apart sugar."  That sugar is composed of 6 carbon atoms, 12 hydrogen atoms and 6 oxygen atoms.  The end products of glycolysis are 2 3-carbon molecules of pyruvic acid and 2 molecules of NADH (NAD+ that has been reduced).  Glycolysis occurs by way of substrate level phosphorylation, which means that a phosphoryl group is added to adenosine diphosphate (ADP) to create adenosine triphosphate (ATP).  Because glycolysis needs 2 ATP to begin and because 4 ATP are created, there is a net gain of 2 ATP.

From glycolysis, pyruvic acid is oxidized to create acetyl CoA.  This means that electrons are taken from pyruvic acid and it loses a carboxyl group.

Then, acetyl CoA moves into the Krebs cycle which occurs in the matrix of the mitochondria.  Here, the carbons in acetyl CoA are released as CO2.  The energy from those released bonds is held as NAD+ and FAD.  Throughout the Krebs cycle NAD+ and FAD are combined with hydrogen electrons to produce NADH and FAD+, respectively.  The added electrons are then passed through the electron transport chain.  A total of 2 ATP are produced by the Krebs cycle.

The electron transport chain is located in the cristae of the mitochondria.  Here, the extra electrons of NADH and FAD+ are passed down a series of transporters.  These transporters are arranged in order from higher affinity for electrons to lower affinity for electrons.  As the electrons are passed from one transporter to another, protons are pumped across the cristae.  This is called a chemiosmotic mechanism.  It creates an energy potential that is collected by ATP synthase and converts ADP to ATP.  The process is called oxidative phosphorylation.  About 32 ATP are created here.

Glycolysis produces 2 ATP, the Krebs cycle produces 2 ATP and the electron transport chain produces 32 ATP for a total of 36 ATP in eukaryotic cells.

At least I hope so, because this is what I'm putting on my first microbiology test in 4 hours.